rabbit anti rat pdgfrbeta Search Results


rabbit anti-rat pdgfrβ 380,772  (ZenBio)
90
ZenBio rabbit anti-rat pdgfrβ 380,772
a The transcriptomic profile of typically upregulated angiogenesis-associated genes in tumor tissues derived from HCC patients. b Protein expression profiles of angiogenesis-associated genes that were correlated with the severity of HCC. c H&E and <t>PDGFRβ</t> staining of liver tissues derived from HCC patients or residual normal liver tissues of the donor liver for transplantation. PDGFRβ-positive cells (red) were visualized by antibodies against human PDGFRβ. The nuclei of the cells were stained by DAPI (blue). d Western blot of PDGFRβ in normal or HCC tissues ( n = 3)
Rabbit Anti Rat Pdgfrβ 380,772, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a The transcriptomic profile of typically upregulated angiogenesis-associated genes in tumor tissues derived from HCC patients. b Protein expression profiles of angiogenesis-associated genes that were correlated with the severity of HCC. c H&E and PDGFRβ staining of liver tissues derived from HCC patients or residual normal liver tissues of the donor liver for transplantation. PDGFRβ-positive cells (red) were visualized by antibodies against human PDGFRβ. The nuclei of the cells were stained by DAPI (blue). d Western blot of PDGFRβ in normal or HCC tissues ( n = 3)

Journal: European Journal of Nuclear Medicine and Molecular Imaging

Article Title: Preclinical evaluation of 68 Ga-radiolabeled trimeric affibody for PDGFRβ-targeting PET imaging of hepatocellular carcinoma

doi: 10.1007/s00259-023-06260-x

Figure Lengend Snippet: a The transcriptomic profile of typically upregulated angiogenesis-associated genes in tumor tissues derived from HCC patients. b Protein expression profiles of angiogenesis-associated genes that were correlated with the severity of HCC. c H&E and PDGFRβ staining of liver tissues derived from HCC patients or residual normal liver tissues of the donor liver for transplantation. PDGFRβ-positive cells (red) were visualized by antibodies against human PDGFRβ. The nuclei of the cells were stained by DAPI (blue). d Western blot of PDGFRβ in normal or HCC tissues ( n = 3)

Article Snippet: To detect the colocalization of PDGFRβ and Z TRI in tumor vessels, HCC tissues were sectioned as 6-μm slices under frozen conditions, followed by incubation with goat anti-human PDGFRβ (AF385; R&D, MN), rabbit anti-rat PDGFRβ (380,772; ZenBio, Chengdu, China), or rat anti-human CD31 (303,102, BioLegend, CA; Clone:WM59) at 37 °C for 1.5 h. Subsequently, the tissues were washed with PBS and incubated with corresponding secondary antibodies (BioLegend, CA) for an additional 0.5 h prior to observation under a Zeiss LSM800 laser scanning confocal microscope (Zeiss, Germany).

Techniques: Derivative Assay, Expressing, Staining, Transplantation Assay, Western Blot

Z TRI shows high affinity for PDGFRβ. a Affinity of Z MON and Z TR affibody to PDGFRβ analyzed by biolayer interferometry at different concentrations. b Binding of Z TRI to PDGFRβ. + pericyte was evaluated by flow cytometry. c Co-localization of Z TRI (green) with PDGFRβ (red) in normal and HCC clinical specimens. The nuclei of cells were visualized by DAPI staining (blue)

Journal: European Journal of Nuclear Medicine and Molecular Imaging

Article Title: Preclinical evaluation of 68 Ga-radiolabeled trimeric affibody for PDGFRβ-targeting PET imaging of hepatocellular carcinoma

doi: 10.1007/s00259-023-06260-x

Figure Lengend Snippet: Z TRI shows high affinity for PDGFRβ. a Affinity of Z MON and Z TR affibody to PDGFRβ analyzed by biolayer interferometry at different concentrations. b Binding of Z TRI to PDGFRβ. + pericyte was evaluated by flow cytometry. c Co-localization of Z TRI (green) with PDGFRβ (red) in normal and HCC clinical specimens. The nuclei of cells were visualized by DAPI staining (blue)

Article Snippet: To detect the colocalization of PDGFRβ and Z TRI in tumor vessels, HCC tissues were sectioned as 6-μm slices under frozen conditions, followed by incubation with goat anti-human PDGFRβ (AF385; R&D, MN), rabbit anti-rat PDGFRβ (380,772; ZenBio, Chengdu, China), or rat anti-human CD31 (303,102, BioLegend, CA; Clone:WM59) at 37 °C for 1.5 h. Subsequently, the tissues were washed with PBS and incubated with corresponding secondary antibodies (BioLegend, CA) for an additional 0.5 h prior to observation under a Zeiss LSM800 laser scanning confocal microscope (Zeiss, Germany).

Techniques: Binding Assay, Flow Cytometry, Staining

a Schematic illustration for the preparation of [ 68 Ga]Ga-DOTA-Z TRI . Z TRI affibody was conjugated with DOTA-NHS ester followed by radiolabeling with 68 Ga. b Stability of [ 68 Ga]Ga-DOTA-Z TRI in PBS and FBS for 6 h in vitro. c Cellular binding assay of [ 68 Ga]Ga-DOTA-Z TRI to PDGFRβ + pericytes. d Autoradiography of [ 68 Ga]Ga-DOTA-Z TRI and PDGFRβ immunofluorescent staining in human liver tissues with or without HCC

Journal: European Journal of Nuclear Medicine and Molecular Imaging

Article Title: Preclinical evaluation of 68 Ga-radiolabeled trimeric affibody for PDGFRβ-targeting PET imaging of hepatocellular carcinoma

doi: 10.1007/s00259-023-06260-x

Figure Lengend Snippet: a Schematic illustration for the preparation of [ 68 Ga]Ga-DOTA-Z TRI . Z TRI affibody was conjugated with DOTA-NHS ester followed by radiolabeling with 68 Ga. b Stability of [ 68 Ga]Ga-DOTA-Z TRI in PBS and FBS for 6 h in vitro. c Cellular binding assay of [ 68 Ga]Ga-DOTA-Z TRI to PDGFRβ + pericytes. d Autoradiography of [ 68 Ga]Ga-DOTA-Z TRI and PDGFRβ immunofluorescent staining in human liver tissues with or without HCC

Article Snippet: To detect the colocalization of PDGFRβ and Z TRI in tumor vessels, HCC tissues were sectioned as 6-μm slices under frozen conditions, followed by incubation with goat anti-human PDGFRβ (AF385; R&D, MN), rabbit anti-rat PDGFRβ (380,772; ZenBio, Chengdu, China), or rat anti-human CD31 (303,102, BioLegend, CA; Clone:WM59) at 37 °C for 1.5 h. Subsequently, the tissues were washed with PBS and incubated with corresponding secondary antibodies (BioLegend, CA) for an additional 0.5 h prior to observation under a Zeiss LSM800 laser scanning confocal microscope (Zeiss, Germany).

Techniques: Radioactivity, In Vitro, Cell Binding Assay, Autoradiography, Staining

a Biodistribution of [ 68 Ga]Ga-DOTA-Z TRI in BALB/c mice. Mice were intravenously injected with 740 kBq of [ 68 Ga]Ga-DOTA-Z TRI followed by collection the majority organs at 5 min, 15 min, 30 min, 1 h, 2 h, and 4 h post-injection ( N = 5). Red arrow points out the necrotic lesion and the yellow arrow points out the nodule lesion. b PET/CT imaging of [ 68 Ga]Ga-DOTA-Z TRI in DEN-induced primary HCC rats and healthy ones. Rats were intravenously injected with 5.55 MBq of [ 68 Ga]Ga-DOTA-Z TRI and scanned at 1 h post-injection. Tumor lesion was pointed out by red arrows. The corresponding liver tissues were collected and [ 68 Ga]Ga-DOTA-Z TRI uptake was investigated by autoradiography after scanning. H&E staining was used to identify the structure of tumor tissues, while AFP immunohistochemical staining and PDGFRβ immunofluorescent staining were performed as index of HCC

Journal: European Journal of Nuclear Medicine and Molecular Imaging

Article Title: Preclinical evaluation of 68 Ga-radiolabeled trimeric affibody for PDGFRβ-targeting PET imaging of hepatocellular carcinoma

doi: 10.1007/s00259-023-06260-x

Figure Lengend Snippet: a Biodistribution of [ 68 Ga]Ga-DOTA-Z TRI in BALB/c mice. Mice were intravenously injected with 740 kBq of [ 68 Ga]Ga-DOTA-Z TRI followed by collection the majority organs at 5 min, 15 min, 30 min, 1 h, 2 h, and 4 h post-injection ( N = 5). Red arrow points out the necrotic lesion and the yellow arrow points out the nodule lesion. b PET/CT imaging of [ 68 Ga]Ga-DOTA-Z TRI in DEN-induced primary HCC rats and healthy ones. Rats were intravenously injected with 5.55 MBq of [ 68 Ga]Ga-DOTA-Z TRI and scanned at 1 h post-injection. Tumor lesion was pointed out by red arrows. The corresponding liver tissues were collected and [ 68 Ga]Ga-DOTA-Z TRI uptake was investigated by autoradiography after scanning. H&E staining was used to identify the structure of tumor tissues, while AFP immunohistochemical staining and PDGFRβ immunofluorescent staining were performed as index of HCC

Article Snippet: To detect the colocalization of PDGFRβ and Z TRI in tumor vessels, HCC tissues were sectioned as 6-μm slices under frozen conditions, followed by incubation with goat anti-human PDGFRβ (AF385; R&D, MN), rabbit anti-rat PDGFRβ (380,772; ZenBio, Chengdu, China), or rat anti-human CD31 (303,102, BioLegend, CA; Clone:WM59) at 37 °C for 1.5 h. Subsequently, the tissues were washed with PBS and incubated with corresponding secondary antibodies (BioLegend, CA) for an additional 0.5 h prior to observation under a Zeiss LSM800 laser scanning confocal microscope (Zeiss, Germany).

Techniques: Injection, Positron Emission Tomography-Computed Tomography, Imaging, Autoradiography, Staining, Immunohistochemical staining

PET/CT imaging of [ 68 Ga]Ga-DOTA-Z TRI in rhesus monkeys. a Representative PET imaging of rhesus monkey with primary HCC. Monkeys were intravenously injected with a dose of 3.7 MBq/kg of [ 68 Ga]Ga-DOTA-Z TRI , [ 68 Ga]Ga-DOTA-Z MON , or [ 18 F]-FDG, respectively. Tumor lesion is indicated by red arrows. b Paired comparison of HCC monkey and healthy monkey. Uptake of [ 68 Ga]Ga-DOTA-Z TRI in liver and tumor were evaluated using fused PET/CT imaging and autoradiography. H&E staining and AFP immunohistochemical staining were performed to identify whether the tissue is cancerous. Co-localization of Z TRI (green) with PDGFRβ (red) in collected liver tissues was verified by immunofluorescent staining. The nuclei of cells were visualized by DAPI staining (blue)

Journal: European Journal of Nuclear Medicine and Molecular Imaging

Article Title: Preclinical evaluation of 68 Ga-radiolabeled trimeric affibody for PDGFRβ-targeting PET imaging of hepatocellular carcinoma

doi: 10.1007/s00259-023-06260-x

Figure Lengend Snippet: PET/CT imaging of [ 68 Ga]Ga-DOTA-Z TRI in rhesus monkeys. a Representative PET imaging of rhesus monkey with primary HCC. Monkeys were intravenously injected with a dose of 3.7 MBq/kg of [ 68 Ga]Ga-DOTA-Z TRI , [ 68 Ga]Ga-DOTA-Z MON , or [ 18 F]-FDG, respectively. Tumor lesion is indicated by red arrows. b Paired comparison of HCC monkey and healthy monkey. Uptake of [ 68 Ga]Ga-DOTA-Z TRI in liver and tumor were evaluated using fused PET/CT imaging and autoradiography. H&E staining and AFP immunohistochemical staining were performed to identify whether the tissue is cancerous. Co-localization of Z TRI (green) with PDGFRβ (red) in collected liver tissues was verified by immunofluorescent staining. The nuclei of cells were visualized by DAPI staining (blue)

Article Snippet: To detect the colocalization of PDGFRβ and Z TRI in tumor vessels, HCC tissues were sectioned as 6-μm slices under frozen conditions, followed by incubation with goat anti-human PDGFRβ (AF385; R&D, MN), rabbit anti-rat PDGFRβ (380,772; ZenBio, Chengdu, China), or rat anti-human CD31 (303,102, BioLegend, CA; Clone:WM59) at 37 °C for 1.5 h. Subsequently, the tissues were washed with PBS and incubated with corresponding secondary antibodies (BioLegend, CA) for an additional 0.5 h prior to observation under a Zeiss LSM800 laser scanning confocal microscope (Zeiss, Germany).

Techniques: Positron Emission Tomography-Computed Tomography, Imaging, Injection, Comparison, Autoradiography, Staining, Immunohistochemical staining